anti-cd27-pe-cy7  (Thermo Fisher)

Journal: iScience

Article Title: Tumor-derived colorectal cancer organoids induce a unique Treg cell population by directing CD4 + T cell differentiation

doi: 10.1016/j.isci.2025.111827

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Article Snippet: Murine CD4 + T cells were stained with the following antibodies: anti-CD4-APC (BioLegend), anti-CD25-Pacific blue (BioLegend), anti-OX40-PE (eBioscience), anti-CD27-PE-Cy7 (eBioscience), anti-GITR-PE-Cy7 (BioLegend), and anti-FAS-BV605 (BD optibuild) in MACs buffer for 15 min at RT.

Techniques: Recombinant, SYBR Green Assay, cDNA Synthesis, Enzyme-linked Immunosorbent Assay, Staining, RNA Sequencing, Transgenic Assay, Software, Cell Culture, Membrane, Sterility, Pore Size

Journal: Cell Reports

Article Title: Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies

doi: 10.1016/j.celrep.2024.114811

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Article Snippet: Anti-human CD27-PE-Cy7 , eBioscience , Cat# 25-0271-82; RRID: AB_1724035.

Techniques: Recombinant, Virus, Control, Labeling, Amplification, Binding Assay, Clone Assay, Transfection, Gel Extraction, Polymerase Chain Reaction, Nested PCR, Sequencing, Expressing, Plasmid Preparation, Software, Transmission Assay, Microscopy

Journal: Frontiers in Immunology

Article Title: Isolation and expansion of pure and functional γδ T cells

doi: 10.3389/fimmu.2024.1336870

Figure Lengend Snippet: Antibodies.

Article Snippet: Mouse anti human CD27 PE-Cy7 , Invitrogen , 25-0279-42 , 1:200.

Techniques:

Journal: Frontiers in Immunology

Article Title: Isolation and expansion of pure and functional γδ T cells

doi: 10.3389/fimmu.2024.1336870

Figure Lengend Snippet: Cell count and fold expansion of Vδ2 + T cells after 14 days of expansion. The cells were isolated through mouse anti-human Vδ2 TCR and anti-mouse IgG bead MACS enrichment, with or without additional FACS sort, and expanded straight after isolation or previously expanded Vδ2 + T cells were submitted to a new round of expansion. Expanded cells were stained for CD27 and CD45RA to determine their differentiation status. (A) Representative flow cytometry plots and gating strategy showing the percentage of Vδ2 + T cells in unsorted PBMCs, after Vδ2 TCR specific MACS isolation and after an additional FACS sort for Vδ2 + T cells. (B) The number of Vδ2 + T cells used at the start of expansion, 15.000 and 150.000, and the number of cells yielded after the 14-day expansion ( n=3 ). (C) The fold expansion of the Vδ2 + T cells for each donor of day 7, 10 and 14 relative to the start numbers, 15.000 and 150.0000, respectively ( n=3 ). (D) Flow cytometry plots showing the percentage of the Vδ2 + cells after isolation (top row) using only the anti-Vδ2 TCR antibody combined with anti-mouse IgG beads and MACS separation, without FACS sort, and after a 14-day expansion period (bottom row) for four donors. (E) The percentage of Vδ2 + T cells after 7, 10 and 14 days of expansion and (F) the percentage of CD3 + Vδ2 - T cells and CD3 + Vδ2 + T cells before and after 14 days of expansion. (G) Representative flow cytometry plots showing the frequency of CD3 + Vδ2 - and CD3 + Vδ2 + T cells in PMBCs ( left plot ) and the frequency of naïve (T naive , CD27 + CD45RA + ), terminally differentiated Effector Memory RA (T EMRA , CD27 - CD45RA + ), Effector Memory (T EM , CD27 - CD45RA - ) and Central Memory (T CM , CD27 + CD45 - ) T cells for the CD3 + Vδ2 - ( middle plot ) and CD3 + Vδ2 + T cells ( right plot ) in PMBCs before isolation. (H) Summary of the percentage of T naive , T EMRA , T EM and T CM subsets in CD3 + Vδ2 + T cells before isolation, as determined in (H, I) Flow cytometry plots of three donors showing the distribution of the T naive , T EMRA , T EM and T CM subsets in the total Vδ2 + T cell population after a 14-day expansion culture. Gating is based on freshly isolated CD3 + cells in total PMBCs from a reference donor. (J) Summary of the data in (I, K) The fold expansion of Vδ2 + T cells that were expanded for 14 days that were, straight from culture, submitted to a new expansion culture of 14-days ( n=4 ). (L) Fold expansion of Vδ2 + T cells that were previously expanded for 14 days after which they were cryopreserved for at least four weeks, thawed and submitted to a new expansion culture of 14 days ( n=4 ). The data is shown as the mean and standard deviation of the donors and data from each donor represents the mean of triplicates. Data was analyzed by a one-way ANOVA followed by Tukey’s multiple comparisons test (B, C, F, H, J, K) or a student’s T-test (L) .

Article Snippet: Mouse anti human CD27 PE-Cy7 , Invitrogen , 25-0279-42 , 1:200.

Techniques: Cell Counting, Isolation, Staining, Flow Cytometry, Standard Deviation